Event Date
Event Date
Recent advances in widefield mid-infrared photothermal (MIP) imaging have enabled rapid chemical imaging over fields of view ranging from 20 to 100 µm [1]. We present implementations that significantly extends the FOV beyond 130 micrometers. The widefield setup employed time-gated detection using a tunable pulsed mid-IR quantum cascade laser as the pump, a visible LED as the probe, and a CMOS camera triggered by a waveform generator. Photothermal imaging of tuberculosis-infected lung tissue was performed using an expanded QCL beam, capturing a 6×6 mosaic to extend the 30 µm FOV to over 130 µm after processing. Moreover, our widefield microscope was coupled to a free-electron laser to image tissue sections at ca. 20 times larger FOV per image. [1] Teng, Xinyan, et al. "Mid-infrared Photothermal Imaging: Instrument and Life Science Applications." Analytical Chemistry 96.20 (2024): 7895-7906.
Presenter
Anooj Thayyil Raveendran
Leibniz-Institut für Photonische Technologien e.V. (Germany)
Anooj Thayyil Raveendran is a PhD student in the Spectroscopy and Imaging research group at Leibniz IPHT, Jena, Germany. He earned his MSc in Scientific Instrumentation from the University of Applied Sciences Jena. His doctoral research focuses on photothermal mid‑infrared microscopy for biomedical imaging.