Event Date
Event Date
Collagen's autofluorescence enables label-free tissue imaging and disease detection, but incomplete spectral characterization limits practical applications. We developed an optical parametric oscillator-based fluorescence lifetime imaging system (OPO-FLIm) to comprehensively characterize excitation-emission matrices (EEMs) and lifetimes of collagen types I-V from various organ sources and hydration states. Using 315-405 nm excitation and 350-500 nm emission detection, we found fluorescence lifetimes ranged between 2.9-5.3 ns across all collagen types. Emission spectra red-shifted with longer excitation wavelengths. Hydrated collagen showed broader EEMs, red-shifted emission, and shorter lifetimes than dry collagen. Collagen I and V exhibited similar properties (3.3-5.3 ns), while collagen II showed longer lifetimes (3.9-5.4 ns) and collagen IV showed the narrowest EEMs. Identical collagen types from different sources showed distinct optical signatures. Photobleaching reduced fluorescence intensity but preserved lifetimes. This comprehensive characterization provides critical reference data for biological research and biomedical imaging applications.
Presenter
Univ. of California, Davis (United States)
Xuhui Liu currently works as a postdoc at Dr. Laura Marcu's Lab at UC Davis. His research focuses on the development of biophotonic instruments such as multi-model fluorescence lifetime instruments, FLIm microscopy, multi-spectral FLIm, FLIm-MRI Augmentation, Xuhui's research also focuses on near-infrared spectroscopy/imaging, diffuse speckle contrast analysis, diffuse correlation spectroscopy, and laser speckle contrast imaging.