Event Date
Event Date
Ultracompact ultra-lightweight two-photon (2P) fiberscopes enable real-time functional neuroimaging in freely-behaving rodents, allowing investigation of how head-mounted weight and rotational resistance affect neuronal activity. We developed a levitation system to counteract head-mounted weight and a proactive optoelectrical commutator with real-time torque sensing and feedback control to eliminate rotational constraints. Neuroimaging revealed that both factors influence the overall activity of a non-negligible population of neurons, highlighting the need to study neural networks under near-natural conditions. Furthermore, we demonstrated a focus-tunable 2P fiberscope integrating a miniature compound deformable lens, expanding the capability of 2P fiberscopy in neuroscience and potential clinical applications.
Presenter
Johns Hopkins Univ. (United States)
Dr. Xingde Li is a Professor in the Department of Biomedical Engineering at Johns Hopkins University. His research centers on advanced biophotonics imaging technologies and their applications in both basic and translational research. He has published over 155 journal papers, which have been cited more than 24,000 times, with an H-index of 68. In addition to research, Dr. Li has served on many scientific society committees and held editorial roles as topical editor and associate editor for multiple journals. He is the lead founding EiC of BMEF, a Science Partner Journal. Dr. Li is a Fellow of OPTICA, SPIE, and AIMBE.