Event Date
Event Date
Miniaturized two-photon microscopes (2P Miniscopes) are powerful optical tools that enable functional recording of neural activity at cellular resolution in freely-moving animals. We present a 2P Miniscope which uses an elliptically shaped point-spread-function (PSF) at the sample and reduces the number of raster scanning rows needed to construct each image frame. This strategy increases the imaging speed by >4× compared to point scanning approaches while maintaining cellular resolution. We employ temporal focusing to further constrain the tail of the elliptical PSF in the axial direction. Using our miniscope, we performed in vivo volumetric calcium imaging in mouse cortex while mice were free to behave in an open field. Our new miniaturized two-photon microscope can record neural activity over a large 3D brain volume at a high speed and will be important to study large-scale neuronal circuits during natural mouse behaviors.
Presenter
Ben Mattison
Univ. of California, Davis (United States)
Ben is a PhD candidate in Biomedical Engineering at the University of California, Davis, advised by Prof. Weijian Yang. He works on developing new microscopy tools for neuroscience and is broadly interested in advancing optical methods to tackle biological problems. Prior to UC Davis, he obtained a B.A.Sc. in Engineering Physics from the University of British Columbia.